Root exudates of oilseed rape were collected from the plants in water culture, and determined to contain 16 kinds of amino acids with high performance liquid chromatography (HPLC). Chemotaxis of Bacillus subtilis Tu-100 toward the 16 amino acids, oilseed rape roots and sclerotia, were determined separately with the beaker method and petri dish method. Results show that Tu-100 displayed obvioous chemotaxis toward histidine, proline, arginine, aspartic acid and glutamic acid only, and histidine in particular, and toward oilseed rape roots and sclerotia as well; more obvious toward the former than the latter.