An incubation experiment was conducted to evaluate the effects of temperatureand soil types on soil net nitrification rate. Four temperatures (15, 20, 25, and 30℃) were set, and three types of soils (black soil, chao soil, and red soil) with high and low content of soil organic matter (SOM) were selected. Urea N was applied at an equivalent rate of 200 mg kg^-1 soil. All soil nitrification rates increased along with incubation temperature within the range of 15~30℃, and the relationship between accumulated soil NO₃-N contents and accumulation temperature of incubation (T×t, ℃×d) could be fitted by a single exponential model. Under the same incubation temperature, the soil nitrification rates decreased in a sequence of: chao soil with high SOM > chao soil with low SOM >black soil with high SOM > black soil with low SOM > red soil with low SOM > red soil with high SOM. There was a significant relationships between soil nitrification rate (incubated under 25℃ for 14 days) and soil total phosporus (TP), total potassium (TK), clay content and pH. Soil pH and TP content was the key factors controlling nitrification rate ( R ²=0.981).