Abstract:Brasilobates spinosus Fujita collected from pollution-free soil by Tullgern method was used as subject in an experiment to explore effect of phoxim on activities of enzymes (acetylcholinesterase, adenosinetriphosphatase, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase and catalase) in B. spinosus Fujita, 48 h and 96 h after treatment with phoxim. Results indicate that the activities of acetylcholinesterase and adenosinetriphosphatase declined obviously with increasing phoxim concentration and elapsing time, while the activities of the other four enzymes showed a reverse trend. A certain dose-effect relationship was found to be in existence between activities of the enzymes and concentration of phoxim, and the findings may serve as reference or basis for use of bio-indicator of phoxim pollution of soils.