Movement and transformation of phosphorus (P) in fertilizer microsites in fluvo-aquic soil were studied through an indoor incubation experiment using soil columns. Results show that fertilizer P did not move far, only 8~14 mm, after 7 and 28 days of transportation. Most of the applied P was fixed by the soil within the radius of 0~2 mm of the site of fertilizer placement. The concentrations of water-extractable P, acid-extractable P and available P decreased rapidly with the increase in distance from the site, showing an exponential curve. P movement occurred mainly within the first 7 days after application and most of the P that moved into the soil was fixed by the soil through adsorption. P application rate significantly affected the movements of various fractions of P within the fertilizer microsite, whereas duration of the incubation had significant effect only on the movement of water-extractable P.