Aging behavior in soil, bioaccumulation and effect on antioxidases in earthworms of pyrene were investigated in the lab. Results indicate that its aging rate was rather high at the initial stage, and then slowed down with the time passing by. Its effect inhibiting earthworms growth was rather significant in the first 60 d, but turned to be insignificant on the 150th day in all the pyrene treatments (p<0.05). The higher the pyrene concentration in the soil, the higher its concentration in the earthworm. However, pyrene concentration was higher in earthworms’ intestines than in the epidermis throughout the entire experiment, suggesting that earthworms accumulate pyrene mainly by ingesting it with soil particles rather than by pyrene penetrating through their epidermis. The concentration of pyrene in earthworms’ epidermis and intestines decreased as the incubation went on, showing a significant difference between the determinations on the 60th day and the 150th day (p<0.05). The effect of pyrene promoting SOD activity in earthworm gradually weakened with pyrene aging on, and turned to be inhibiting SOD activity on the 150th day (p<0.05). Compared with CK, Treatment Pyrene (100 mg kg^-1) was significantly higher in CAT activity in earthworm in the first 60 days (p<0.05), and was found almost the same on the 150th day, while MDA content decreased with pyrene aging on and was found to be much lower on the 150th day in both Treatments Pyrene (50 and 100 mg kg^-1) (p<0.05).