In the field of a long-term experiment on integrated water and nitrogen management in the Fengqiu National Agro-ecological Experiment Station, soils samples were collected from plots subjected to different nitrogen fertilization rates (NR), i.e. 0, 150, 190, 230 and 270 kg hm^-2 per season, separately for analysis of parameters of soil fertility (i.e. soil pH, total N, total P, total K, alkalytic N, available P, available K and organic C). Results show that nitrogen fertilization decreased soil pH, available P and total K, but increased total N, alkalytic N and organic C to a varying degree. Apart from organic C which gradually increased with the increase in NR, no other parameters demonstrated any regular changes with increasing NR. After being subjected to 0, 3, 6 and 10 cycles of drying-wetting alternation and 60 days of incubation, soil samples were analyzed for chemical and biological properties (i.e. ammonium N, nitrate N, dissolved organic C, urease activity, dehydrogenase activity, microbial biomass C and basal soil respiration). Two-way ANOVA demonstrates that the number of drying-wetting cycles (NDW) considerably (p< 0.001) affected ammonium N, nitrate N, inorganic N, dissolved organic carbon, dehydrogenase activity, microbial biomass C and basal soil respiration, and that NR and NDW had no significant interactive effects on soil properties, except for dehydrogenase activity. Irrespective of NR, nitrate N, inorganic N, dissolved organic C, urease activity, dehydrogenase activity and microbial biomass C increased with NDW, while BSR responded reversely. Soil properties variation coefficients in soils under high-rated nitrogen fertilization (> 190 kg hm^-2) were lower, and easier to get cluster. The above findings demonstrate that in soils subjected to drying-wetting alternation, high-rated nitrogen fertilization may help maintain their biochemical stability.