Abstract:In order to study the effects of applying combined amendment (prepared by mixing limestone and sepiolite at a mass ratio of 2:1, LS) on remediation results in heavy metal contaminated paddy soils, a field experiment was conducted in a paddy soil near a mining area in southern Hunan, China. The experiment results indicated that applying 0~1.8 kg m-2 of LS significantly increased soil pH and CEC, and decreased exchangeable contents of soil Pb, Cd, Cu and Zn. With increasing in applying amount of LS, the remediation results became more notable. The reduction of exchangeable Pb, Cd, Cu and Zn was an outcome of rising soil pH, CEC and soil colloid adsorption capacity due to addition of limestone and sepiolite. Meanwhile, applying LS resulted obviously in reducing contents of Pb, Cd and Cu in brown rice of the three tested rice varieties (Hanghuazhan, Fengyou 9, and II You 93 in Chinese system), and the maximum reduction of Pb, Cd and Cu contents in brown rice were 55.8%, 66.9%, and 37.4%, respectively. However, applying LS had no significant effects on Zn contents in brown rice. As a result of applying 1.8 kg m-2 of LS to the paddy soil, the Cd content in brown rice of Fengyou 9 could be decreased to 0.195 mg kg-1, lower than the limits stipulated in the National Standard GB 2762-2012, Maximum Content of Contaminants in Food (0.20 mg kg-1). The reduction of Pb, Cd and Cu accumulation in brown rice was due to LS reduced exchangeable contents of soil heavy metals, and there were significantly positive linear correlations between the exchangeable contents of Pb, Cd, Cu in soil and contents of Pb, Cd, Cu in brown rice in natural logarithm.