【Objective】Selenium (Se) is an essential element. About1 billion people the world over suffer Se deficiency, which may lead to a series of human health disorders. The purpose of this study was to explore pathways of Se transporting to grains at the milking stage of two varieties of rice, Xiushui 48 and S. Andrea, relative to form of Se fed to the crop and to better understand mechanism of the translocation of Se to grains of the plant, so as to improve Se content in rice and breed Se-enriched rice cultivars.【Method】Two cultivars of rice (Oryza sativa L.), Xiushui 48, an Se-enriched cultivar, and S. Andrea, a general one, turn out grains differing sharply or by 3 times in Se content. They were selected for comparison in Se accumulation in grains at the milking stage. Four different species of Se [Selenite, Selenate, Selenomethionine (SeMet) and Se-methyl-selenocysteine (SeMeSeCys) ] were supplied, separately, to excised panicles via stems subjected to girdling or none and flag leaves of intact rice plants of the two cultivars during the milking stage. 【Result】Results show that organic selenium could enter into phloem and then be translocated to grains via stem, while only 30% ~ 50% of the inorganic selenium in the phloem was remobilized and transferred into grains via stem, and the remaining transported through xylem. Sodium selenate could be transported to flag leaves via both phloem and xylem, while sodium selenite went mainly through xylem. When fed with sodium selenite or Se-methyl-selenocysteine, excised panicles with or without the stems girdled did not show much difference in Se concentration in grains between the two varieties. But when fed with sodium selenate and selenomethionine, excised panicles of Xiushui 48 with no stem girdled were much higher in grain Se accumulation capacity than those of S. Andrea, and not much difference was found between the excised panicles of the two varieties with stem girdled. It was also found that Xiushui 48 was more capable than S. Andrea of transporting selenate and selenomethionine from stem to grain. Obviously the former is capable of moving more Se from stem and flag leaf to grain than the latter is, which is probably the direct cause of higher Se concentration in grains of Xiushui 48. The finding also indicates that Xiushui 48 is much more capable (p= 0.05) than S. Andrea of transporting Se-Met and selenate through phloem from stems to grains, and from flag leaves to grains, too. 【Conclusion】Selenium-enriched Xiushui 48 can transport more Se from stems and flag leaves to grains than Non-Se-enriched variety, S. Andrea, which is probably the direct cause of the difference between the varieties of rice in grain selenium content.