To explore impacts of mulching plastic film residue (MPFR) on migration of nitrate nitrogen and ammonium nitrogen in soil and growth of flue-cured tobacco roots. A pot experiment coupled with soil column leaching test and was conducted and had the pots divided into four groups or treatments different in amount of MPFR in the soil, i.e. 0 (CK), 450 kg·hm^-2 (T1), 900 kg·hm^-2 (T2) and 1 350 kg·hm^-2 (T3); contents of soil nitrate nitrogen and ammonium nitrogen in different soil layers were determined, and length, surface area, volume, diameter and number of root tips and dry matter accumulation of flue-cured tobacco were also measured. MPFR in the soil retarded movement of soil nutrient, and hence migration of nitrate nitrogen and ammonium nitrogen in the soil significantly; MPFR inhibited development of flue-cured tobacco roots and affected, morphology parameters of the roots, such as length, surface area and volume, which decreased gradually with increasing amount of MPFR. When MPFR in the soil reached ≥900 kg·hm^-2 in amount, it significant impeded growth of the underground and aboveground parts of the flue-cured tobacco. So, it is quite obviously that mulching plastic residues in the fields will inhibit migration of soil nutrients and growth and development of the flue-cured tobacco therein.