[Objective] Nowadays, more attention should be paid to the situation of agricultural environment and the quality and safety issues of agricultural produces because more and more various pollutants are entering into the agricultural soil. Among the pollutants, heavy metals and antibiotics are two of the most important types of pollutants in the farmland soil. In this study, soil microorganisms are used as indicators to illustrate impacts of combined pollution of heavy metals and antibiotics on soil environment.[Method] An in-lab analogue incubation experiment was conducted in this study, with the soil spiked with doxycycline (DOX, 0, 8 and 15 mg·kg^-1) and copper (Cu, 0, 100 and 400 mg·kg^-1), singly or combinedly, at a varying rate as the representative pollutants of antibiotic and heavy metal, respectively. Effects of the pollutants on respiration of soil microbes, activities of soil enzymes, like urease, sucrase and catalase, and abundance of tet ARGs (tetracycline-resistance genes) were investigated.[Result] Results show that throughout the whole incubation period (30 days), the pollution of DOX and Cu, either singly or combinedly, inhibited significantly respiration of soil microbes in intensity, and activities of sucrase and catalase, but stimulated that of urease, and the effect was apparently catalase than on sucrase. To sum up, the effects of combined pollutions is much higher than that of singe pollutions and the addition of DOX stimulated the initial effect of Cu on respiration of soil microbes or activities of soil enzymes. Correlation analysis shows that the activity of catalase/urease was significantly and negatively related to Cu concentration and urease activity significantly and negatively correlated with sucrase activity. Besides, it was also found that the total relative abundance of four types of antibiotic resistance genes (ARGs) decreased first and then turned backwards, which was probably owing to the tie lag of about 7-15 days the soil system needs to adapt and age after the addition of the pollutants. Compared with other treatments, the addition of high concentrations of Cu and DOX significantly increased the abundance of ARGs at the mid-and late-stages of the incubation, especially the relative abundance of tetA and tetW. The addition of a high concentration (400 mg·kg^-1) of Cu improved the ability of DOX to induce high relative abundance of tet ARGs.[Conclusion] All the findings in this study indicate that combined pollution of Cu and DOX has dramatic impacts on functions of the soil microbial system.