【Objective】Effects of selenium application on soil physical and chemical properties, nutrient elements of Chrysanthemum morifolium cv Hangbai, selenium content and yield of the plant, relative to application method were analyzed.【Method】In this experiment, nano-selenium was used as extraneous selenium for exploration of effect of its application on quality of Chrysanthemum morifolium. In terms of application of selenium, this experiment was designed to have four treatments, i.e. Treatment T (applying selenium as fertilizer into the soil (T); Treatment Y (spraying selenium solution onto leaves), Treatment (T+Y) (applying and spraying selenium together), and Treatment CK (no selenium applied as control.【Result】 Results show that as compared with CK, selenium application, regardless of method, did not have much effect on soil bulk density, soil porosity and soil water content and on the activities of soil alkaline phosphatase and soil catalase, either, but did dull the activity of urease and stimulate that of sucrase significantly in the soil, lower the Vc content and increased the contents of flavonoids and chlorogenic acid and the Se content in the inflorescences of the plant. Besides, selenium application increased the number of flowers per plant, the dry biomass per flower and the yield of flowers per mu. The effect was the most significantly in Treatment T+Y.【Conclusion】Based on comprehensive comparison analysis of the effects of the three selenium application methods on soil physical and chemical properties, nutrient contents selenium content and yield of Chrysanthemum morifolium, it could be concluded that selenium application via both fertilization and foliar spraying is the most effective method for selenium application to Chrysanthemum morifolium. All the findings in the experiment may serve as a practical and theoretical basis for the production of Se-enriched Chrysanthemum.