A field experiment was conducted to explore effects of combined application of organic manure (300 g·kg^-1 or 400 g·kg^-1 rapeseed cake) and chemical fertilizer on yield, quality and nutrient utilization efficiency of flue-cured tobacco, in an attempt to provide scientific basis for improving utilization efficiency of the rapeseed resources and tobacco quality. Using flue-cured tobacco of cultivar K326 as test material, the experiment was designed to have ten fertilization treatments, i.e. T1 (chemical fertilizer, consisting of 400 kg·hm^-2 specialized tobacco fertilizer equal to 60 kg·hm^-2 in N nutrient + 362.5 kg·hm^-2 superphosphate +160 kg·hm^-2 potassium sulfate as control), T2 (OR30 at 400 kg·hm^-2, equal to T1 in N rate ), T3 (OR30 at 265 kg·hm^-2, equal to T1 in N rate, and 150 kg·hm^-2 potassium nitrate,) , T4 (OR30 at 225 kg·hm^-2, 10% less than T1 in N rate, and 150 kg·hm^-2 potassium nitrate), T5 (OR30 at 440 kg·hm^-2, 10% more than T1 in N rate) , T6 (OR40 at 500 kg·hm^-2, equal to T1 in N rate), T7 (OR40 at 331.2 kg·hm^-2 and 150 kg·hm^-2 potassium nitrate, equal to T1 in N rate) , T8 (OR40 at 281.2 kg·hm^-2 and 150 kg·hm^-2 potassium nitrate, 10% less than T1 in N rate) , T9 (OR40 at 550 kg·hm^-2, 10% more than T1 in N rate ) and T10 (neither manure or fertilizer). Results showed that compared with treatment T1, Treatments T2, T3, T5, T7 and T9 significantly improved growth, leaf area and leaf area coefficient of the tobacco plants by 1.74% -4.51% and 4.47%-19.11%, respectively. Compared with treatment T1, Treatments T4, T5, T7 and T9 significantly increased fresh weight, dry weight and root volume of the plants; Treatments T2, T4, T5, T7 and T9 increased by 2.69%-7.46% in yield, increase rate; and Treatments T2, T5 and T9 increased significantly or by 10.99% -13.46% in output. Meanwhile, Treatments T2, T4, T7 and T9 increased contents of total sugar, reducing sugar, potassium oxide and water-soluble chlorine, and decreased the content of total nitrogen and nicotine in the middle position tobacco leaves. Compared with Treatment T1, Treatments T2, T4, T5 ,T7 and T9 were 9.31% -25.17% and 7.67%-22.32% higher to apparent and physiological N use efficiency, respectively; Treatments T2, T4 and T9 were 19.85% -28.05% and 14.71% - 21.39%, respectively higher in apparent and physiological P use efficiency; and Treatments T2, T4, T5 and T9 were 8.55%-17.40% and 9.06% -21.98%, respectively higher in apparent and physiological K use efficiency. In summary, the application of a proper amount of complex organic-inorganic fertilizer in Treatments T5 and T9, and the application plus potassium nitrate in Treatments T4 and T7 can promote growth, yield, output value and quality of the flue-cured tobacco by improving their NPK absorption.