Succession of Microbial Community in Typical Black Soil under Different Land Use Pattern
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S154.38+1

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    Abstract:

    【Objective】To explore the succession of soil microbial community structure and its environmental driving mechanism under different land use pattern, soil samples were collected from plots in a long-term(32 years)field experiment station in the black soil region of Northeast China.【Method】 The long-term field experiment was started on different land usage in the National Field Research Station of Agro-Ecosystem of Chinese Academy of Sciences in 1985. The experiment was designed with three treatments and three replicates, i.e. bareland(vegetation removed), cropland(crop rotation without fertilizer), and grassland(natural meadow vegetation). Soil samples were collected for analysis from the 0~20 cm soil layer in October 2017. Soil microbe was determined by the Illumina high-throughput sequencing technology on MiSeq platform. 【Result】The soil organic matter was 52.07 g·kg-1, 54.83 g·kg-1 and 61.54 g·kg-1 in the treatments of bareland, cropland and grassland, respectively. Compared to the bareland, the soil organic matter increased by 8.0% and 27.5% in the cropland and grassland, respectively. The available nitrogen, phosphorus and potassium also increased significantly in cropland and grassland. At the same time, the total abundance of microbe increased from 2.25×107 copies·g-1 dry soil to 8.08×107 copies·g-1 dry soil and 1.69×108 copies·g-1 dry soil in the three treatments, respectively. The abundance of microbe increased by 2.58 times and 6.51 times in cropland and grassland, respectively. At the phylum level, dominant microbe were Proteobacteria, Actinobacteria, and Acidobacteria, whose relative abundances were more than 19% and with no significant differences in the three treatments(P > 0.05). The 54 of 228 microbial genera detected showed significant differences(P < 0.05)among the three treatments, and most of them showed relatively low abundance. The genera of Gemmatimonas, Rhodoplanses, Arenimonas showed significant differences and higher abundance in the three treatments. The redundancy analysis showed that soil properties were the main environmental driving force for the differentiation of microbial community structure, including cation exchange capacity, total nitrogen, organic matter, available nitrogen, available phosphorus and available potassium. Network analysis showed that Granulicella had the strongest association with soil properties and other microbial genera, suggesting that this genus can be used as an indicator to assess changes in the black soil. 【Conclusion】 The vegetation cover was an key factor for the succession of soil microbial communities. Research should thoroughly explore the functions and agricultural environmental significance of microbe under in-situ conditions in black soil in the future, to provide theoretical references for maintaining the virtuous cycle of nutrient and developing sustainable agricultural ecological management models.

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HU Fang, WANG Fang, HAN Xiaozeng, XU Min, FU Yuhao, YAN Jun, JIA Zhongjun, TIEDJE James M., JIANG Xin. Succession of Microbial Community in Typical Black Soil under Different Land Use Pattern[J]. Acta Pedologica Sinica,2022,59(5):1238-1247.

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History
  • Received:March 04,2021
  • Revised:May 12,2021
  • Adopted:
  • Online: August 16,2022
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