【Objective】 This study aimed to elucidate the characteristics and correlations between Fusarium oxysporum and soil nutrients in banana orchards with Panama wilt occurring through analyzing the number of F. oxysporum and nutrient contents in soils. This can provide a certain theoretical basis for the prevention and control of banana Panama wilt.【Method】 Nine symptomatic and nine non-symptomatic banana plants in each orchard from 15 banana orchards in the main banana production areas in China and Laos were randomly selected for soil sample collection. Then real-time quantitative PCR and soil agrochemical analysis were used to explore the abundance of F. oxysporum and nutrient contents in the 270 sampled soils.【Result】 The result showed that the abundance of F. oxysporum in the soil sampled near the symptomatic banana roots was significantly higher than that collected near the non-symptomatic banana roots, with an average copy of 5.5 as lg-transformed per gram of dry soil. However, there were almost no significant differences between the nutrient contents of soils sampled from symptomatic and non-symptomatic banana plants. The distribution of various indexes of soil nutrients in the studied banana orchards was different based on each factor. Among them, only the contents of organic matter and total phosphorus were distributed normally while the soil pH, and contents of total nitrogen and zinc were skewed normal distribution. Mantel test results further displayed that the overall soil nutrients were significantly correlated to the abundance of F. oxysporum. Specifically, the soil pH, and contents of iron, manganese, and copper were significantly and negatively correlated to the abundance of F. oxysporum revealed by the Spearman correlation analysis between the abundance of F. oxysporum and each soil nutrient. In addition, the correlation analysis between F. oxysporum and nutrients in soils with different degrees of acidity and different organic matter contents showed that in the weakly acidic banana garden soil (pH > 6.0), the number of F. oxysporum in soils was significantly negatively correlated with the contents of iron, manganese and copper. Meanwhile in soils where organic matter contents were not seriously lacking (> 1%), the abundance of F. oxysporum in soils displayed a significant negative correlation with soil pH and the contents of iron, manganese, copper and zinc.【Conclusion】 In summary, banana orchards in the main banana production areas in China and Laos usually exhibit an acidification trend. Symptomatic banana soil showed an increase of F. oxysporum with a negative correlation to soil pH and the contents of trace elements, especially iron, manganese, copper and zinc.