【Objective】 This study aimed to clarify the effects of fertilizer and organic fertilizer combined with biomass charcoal on the forms of nutrients and nitrogen in rhizosphere/non-rhizosphere soil, which is helpful for the efficient utilization and scientific management of farmland nitrogen.【Method】 Taking the pot experiment of lemon as the research object, this paper set up six treatments:no fertilizer (CK), chemical fertilizer (CF), manure (M), chemical fertilizer with biomass charcoal (CFB), manure with biomass charcoal (MB) and fresh manure with biomass charcoal (FMB). By measuring the changes of nutrient content in the rhizosphere and non-rhizosphere soils and the transformation of soil nitrogen storage forms, the effects of organic fertilizer combined with biomass charcoal on the forms of soil nutrients and nitrogen in rhizosphere and non-rhizosphere were studied.【Result】 The results showed that compared with CK treatment, MB treatment increased non rhizosphere and rhizosphere soil pH by 0.32 and 0.28 units, and FMB treatment increased rhizosphere soil pH by 0.63 units; MB and FMB treatments increased the content of organic matter in rhizosphere soil by 25.4% and 84.9% respectively, and significantly increased the content of total nitrogen in rhizosphere soil by 25.4% and 50.9%, showing obvious rhizosphere effect. Fertilization can significantly increase the content of soil ion-exchange nitrogen (IEF-N), and CF and CFB treatments had the best effect. Applying manure and biomass charcoal can significantly increase the content of carbonate-bound nitrogen (CF-N) in soil, and M and MB treatments are the best, and CF-N has a rhizosphere enrichment effect. The combined application of biomass charcoal (CFB, MB and FMB) can promote the transformation of soil non-convertible nitrogen (NTF-N) to two more active nitrogen forms, iron manganese oxide bound nitrogen (IMOF-N) and organic sulfide bound nitrogen (OSF-N). IMOF-N and OSF-N account for 35.9%-61.7% and 26.7%-46.6% of convertible nitrogen, respectively, and are the main components of rhizosphere and non-rhizosphere soils convertible nitrogen (TF-N).【Conclusion】 Therefore, manure combined with biomass charcoal is an effective way to improve rhizosphere and non-rhizosphere soil nutrients and regulate nitrogen transformation.