【Objective】To evaluate the NO₃^–-N removal capacity of typical farmland profiles in southern China and to explore the hot moments and hot spots of denitrification.【Method】Paddy fields (rice-wheat rotation), vegetable fields, and orchards (vineyard) in the Taihu Lake region were selected for this study. Near in-situ incubation of flooded and non-flooded layers of the soil profile (0-300 cm) of these fields was performed using the Membrane Inlet Mass Spectrometer (MIMS) and Robotized continuous flow incubation system (RoFlow) over a year. 【Result】Our results showed that the soil denitrification rate exhibited distinct hot moments and hot spots across the three planting patterns. Denitrification hot moments in paddy fields were primarily observed in October during the rice season, with a rate of 17.6 nmol·g⁻¹·h⁻¹. The denitrification hot moments of vegetable fields and orchards mainly occurred in March, with rates of 44.2 nmol·g⁻¹·h⁻¹ and 45.3 nmol·g⁻¹·h⁻¹, respectively. The hot spots of denitrification in the paddy fields occurred in the topsoil (0-20 cm) with an average rate of 3.4 nmol·g⁻¹·h⁻¹. The denitrification hot spots of the vegetable fields and orchards mainly occurred at 20-100 cm, with average rates of 11.7 nmol·g⁻¹·h⁻¹ and 9.4 nmol·g⁻¹·h⁻¹, respectively. Also, the removal rate of NO₃^–-N in these denitrification hot spots exceeded 90%, and almost all NO₃^–-N in the soil profile was removed under the three planting patterns. Correlation analysis results indicated that the soil NO₃^–-N content was the primary limiting factor for denitrification. 【Conclusion】Our study reveals that the farmland soil profiles under the three planting patterns in the Taihu Lake area exhibit high denitrification rates with distinct denitrification hot moments and hot spots, effectively removing NO₃^–-N from the soil profile.