Home > Online First
PDF HTML XML Export Cite reminder
The Biocontrol Effect and Microecological Mechanism of Lysobacter enzymogenes Bio-organic Fertilizer on Salvia miltiorrhiza Root Rot
Author:
Affiliation:

Key Laboratory of Organic-Based Fertilizers of China, Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Educational Ministry Engineering Center of Resource-Saving Fertilizers, College of Resources and Environmental Science, Nanjing Agricultural University

Fund Project:

Supported by the National Key Research and Development Program of China (No. 2022YFC3501501), the National Natural Science Foundation of China (Nos. 42090064 and 42377118) and the Natural Science Foundation of Jiangsu Province, China (No. BK20240194)

  • Article
    • | |
  • Metrics
    • | | |
  • Cited by
    • | |
  • Comments
    Abstract:

    【Objective】Salvia miltiorrhiza Bunge, commonly known as Danshen, is a perennial herbaceous plant that is a traditional and widely used medicinal herb in China. Intensive cultivation has led to frequent outbreaks of soil-borne diseases, notably root rot, which significantly limits the yield and quality of Danshen. 【Method】This study compared the antagonistic abilities of four Lysobacter strains isolated from the rhizosphere of Solidago canadensis against the root rot pathogen Fusarium oxysporum 220. To identify the most efficient fermentation substrate for the selected strain, microbial solid-state fermentation was conducted using tobacco stalk, rice straw, sorghum straw, corn straw, and Solidago canadensis straw. Based on the optimal fermentation substrate, a bio-organic fertilizer (BOF) was prepared. Subsequently, pot experiments were conducted to evaluate the effectiveness of this BOF in controlling Danshen root rot. Finally, based on metagenomic sequencing, the key microbial groups and functional genes enriched by BOF were analyzed. 【Result】The results of the study demonstrated that the Lysobacter enzymogenes strain Le395 exhibited strong antagonistic effects against the root rot pathogen F. oxysporum 220. In the organic fertilizer raw material screening experiment, it was found that the optimal fermentation substrate for strain Le395 was Solidago canadensis straw. In the pot experiment, it was observed that the application of the BOF effectively controlled Danshe root rot disease. Compared to the control group (CK), the disease incidence was reduced by 50.3%, and the disease index decreased by 50.2%. Additionally, the BOF showed significant growth-promoting effects. The above-ground fresh biomass of the plants treated with BOF increased by 163.1%, while the below-ground fresh biomass increased by 147.0% compared to the CK group. Finally, qPCR and metagenomics sequencing analyses revealed that the application of Le395 BOF significantly decreased the abundance of Fusarium and F. oxysporum in the rhizosphere of Danshen. It also increased the abundance of Lysobacter and L. enzymogenes, while modulating the rhizosphere microbial community structure and enhancing the abundance of disease resistance- and growth promotion-related functional genes in the rhizosphere. 【Conclusion】These findings provide a theoretical and technical foundation for developing an ecological and sustainable strategy for controlling Danshen root rot using bio-organic fertilizer.

    Reference
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:31
  • PDF: 459
  • HTML: 0
  • Cited by: 0
History
  • Received:June 25,2024
  • Revised:September 20,2024
  • Adopted:November 19,2024
  • Online: November 19,2024
Article QR Code
今日访问量：22 您是第4522125 位访问者
PostalAddress：江苏省南京市北京东路71号 Zip Code：210008 Phone：+86-25-86881237
WebSite：http://pedologica.issas.ac.cn E-mail：actapedo@issas.ac.cn
CopyRight All：《土壤学报》编辑部 Supported by:Beijing E-Tiller Technology Development Co., Ltd. ICP:05004320号-1