【Objective】Microplastic pollution has emerged as a significant global environmental issue, with fibrous microplastics constituting a substantial portion of this pollutant. These tiny fibers, often derived from synthetic textiles and industrial processes, can infiltrate various ecosystems, including soil and water. Understanding the behavior and fate of fibrous microplastics in plants is crucial to assessing their potential ecological risks and human health implications. This study aimed to investigate the uptake and transport of polyacrylonitrile (PAN) fibrous microplastics in corn plants, a widely cultivated crop. 【Method】To achieve this, PAN microfibers, with an average diameter of 200 nm and an aspect ratio of 20±5 were synthesized using electrostatic spinning and fluorescently labeled to facilitate tracking. Corn seedlings were exposed to these labeled fibers under hydroponic conditions for two weeks. The distribution and localization of the fibers within the plant tissues were subsequently examined using laser confocal microscopy and scanning electron microscopy. 【Result】The results revealed that the PAN microfibers were able to penetrate the root interior through nascent lateral root fissures. Once inside the root, the fibers were transported upward along the xylem ducts to the stems. However, the fibers were not detected in the vascular tissues of the leaves, suggesting that their translocation was primarily restricted to the root and stem systems. The ability of corn roots to absorb and accumulate these fibers highlights the potential for microplastic bioaccumulation in plant tissues. Also, the large surface area of fibrous microplastics may have contributed to their efficient adsorption by root surfaces, facilitating their entry into the plant whereas the fibrous morphology enhanced their penetration through root tissues. In addition, the accumulation of fibrous microplastics in plants could potentially disrupt plant growth, development, and physiological processes. 【Conclusion】This study provides the first direct evidence of fibrous microplastic uptake and transport in higher plants, and highlights the possible transfer of fibrous microplastics from plants to herbivores and humans through the food chain. The research results provide an important scientific basis for in-depth understanding of the migration and transformation laws of different shapes of microplastics in plant-soil systems, and also provide support for an assessing their impact on ecological environmental health and food safety. Our results necessitate the need for further research to investigate the factors influencing fibrous microplastic uptake and transport in plants, the potential ecological and toxicological impacts of fibrous microplastic exposure, and effective strategies for minimizing their environmental and human health risks.