【Objective】Anoxic microsites significantly inhibit the loss of soil organic carbon (SOC). Soil aggregates, as potential sites for the development of anoxic microsites, are closely related to the accumulation of SOC, yet few studies have investigated the impact of anoxic microsites on SOC within soil aggregates.【Method】This study collected soil samples from four types of ecological restoration and employed soil incubation and gas chromatography to calculate the extent of anoxic protection. The dry sieving method was used to obtain inner and outer soil samples from macroaggregates, and the abundance of anoxic microsites and organic matter components was compared.【Result】The results showed that natural shrubland and grassland had higher extents of anoxic protection, at 33.5% and 36%, respectively, followed by planted forests at 15.9%, and croplands had the lowest at -8.9%. The inner layers of macroaggregates had higher Fe²⁺ content, a greater abundance of anoxic microsites, and higher relative contents of aromatic hydrocarbons, lipids, and lignin. Soil respiration rate was significantly negatively correlated with the extent of anoxic protection.【Conclusion】The results indicate that a stable soil physical environment is crucial for the effectiveness of anoxic protection. Anoxic microsites selectively protect reduced organic matter in the inner layers of macroaggregates and, to some extent, inhibit the loss of SOC, providing new insights into soil carbon cycling and carbon sink functions.