【Objective】The rhizosphere is the interface between plants and soil, and plants affect the composition of soil microbial communities by secreting root exudates, while rhizosphere microorganisms can affect plant physiology and development through multiple mechanisms. Therefore, the development of accurate and applicable root exudate and soil sample collection methods are very important in understanding the ecological process of plant root-soil interface. However, the in-situ collection of rhizosphere soil and root exudates remains understudied. 【Method】This paper introduced a novel device for the in-situ dynamic visualization and accurate collection of rhizosphere soil and root exudates. The device comprises a plant growth compartment, a root growth compartment, a soil compartment, and a root exudate sampling system. The device is designed by partitions, planting seeds or seedlings in the plant growth compartment, allowing the root system to extend into the lower root growth compartment through a slit at the bottom. Also, the nylon membrane isolates the soil from the root system to ensure a two-way pure exchange. On the one hand, it allows soil nutrients to infiltrate, ensuring normal plant growth, and on the other hand, it prevents particle contamination. At the same time, a multi-interface connected sampling system is set up to realize the accurate partition collection of root exudates. The sampling process is automated, with a vacuum pump controlled by a solenoid switch and a program to regularly rinse and extract secretions into the sampling bottle. For soil sampling, the depth and distance from the root system are flexibly selected, and the soil quantity is estimated by the sampling tube scale to ensure the accuracy and comprehensiveness of the experimental data. For validation, root exudates of wheat during the grain-filling stage were collected by both the device collection method and the solution collection method. The content of quercetin in the root exudates was determined by high-performance liquid chromatography. A standard curve was prepared using quercetin as the standard substance, and the content of quercetin in the root exudates was calculated. 【Result】This device innovatively integrated the functions of plant growth, root exudates sampling and rhizosphere soil collection. It realized the accurate, in-situ, continuous and dynamic collection of plant root exudates and rhizosphere soil through ingenious design, and the collection range could be accurately concentrated in the root system and the soil environment in the same area, so as to ensure the simultaneous collection of exudates and rhizosphere soil. During collection of root exudates, the content of quercetin obtained by the device collection method was close to that by the solution collection method, and it eliminated the cumbersome operations such as root stripping, supported continuous sampling at multiple time points for the same plant, and significantly improved the research efficiency of individual plants. Moreover, this method ensured the normal growth of plants, and there was no interference with soil components, and provided high accuracy. 【Conclusion】This device, with its comprehensive functions, provides efficient sampling capacity and precise control mechanism, and can serve as a powerful tool in the field of plant physiological ecology, and is conducive for promoting the in-depth development of soil microbiology research.