【Objective】This study aimed to investigate the possible contribution of Fe isotopic composition to weathering and pedogenic processes and the formation of Fe-Mn nodules.【Method】Representative soil profiles were established in the warm-temperate, subtropical, and tropical zones in eastern China. The physical and chemical properties, geochemical characteristics, and Fe isotopic compositions of the soils and Fe-Mn nodules were analyzed. 【Result】The results indicated that the degree of pedogenic weathering of the soils from the different climatic zones was mainly controlled by climatic factors, and was also related to the parent materials. The ferromanganese nodules produced in the different climatic zones were all enriched in Fe and Mn, but varied greatly in geochemical characteristics. The ferromanganese nodules developed from tropical basalt have total Fe (Fet) and free Fe (Fed) content of 353.7 mg·g-1 and 293.5 mg·g-1, respectively. These were significantly higher than those of the nodules formed from the Quaternary red clay in the subtropical and the eluviated cinnamon soil in the warm-temperate zone. However, the Fe isotopic composition in the red or cinnamon soils developed from the different climatic zones and different parent materials was similar, and their δ56Fe value varied between 0.018‰ and 0.120‰, all close to the baseline of the continental crust igneous rock. In contrast, the δ56Fe value of the ferromanganese nodules formed in the three climatic zones was all negative, with an average of -0.330‰, which was significantly enriched in light Fe isotopes. 【Conclusion】These results showed that there was a trend of lightening in Fe isotopes of the ferromanganese nodules from the tropical to the subtropical to the warm-temperate zones.