Pot experiment and water culture were used to compare the characters of rape varieties. A three-year fix-location field experiment of B fertilizer application was conducted to study the quantity of absorption and accumulation of B during different growing stages of rape. Microscopy was used to detect formation of pollen grain, and ¹⁰B α-solid technique was used to determine the contents and distribution of B in the flower organs of rape. The results showed that responses of rape vaneties to B nutrition varied greatly.