The effects of combined application of organic manure and fertilizers on senescences of root and flag leaf in winter wheat (Triticum aestivum L.) were studied through experiment with soil column culture. The results showed that the combined application of organic manure and fertilizers resulted in a decrease of lipid peroxidation and an increase of superoxide dismutase (SOD) activity in root and flag leaf after anthesis, increased the root vigour and dry root weight especially in 40-100cm soil layer, reduced the decline rate of photosynthetic rate of flag leaf, delayed the senescences of root and flag leaf, increased the grain weight, biomass, economic index and grain yield.