In order to explore potential of the variation of GSH and MDA levels in Eisenia fetida as bio-indicators in monitoring heavy metal contamination of soil,effect of copper on Eisenia fetida was studied using artificial soil and contaminated agricultural soil.Results show that the levels of GSE and MDA in E.fetida are significantly related to copper in the artificial soil,with correlation coefficients being 0.556,0.807 and 0.775 between Cu concentration and levels of GSH and 0.760,0.672 and 0.544 between Cu concentration and levels of MDA,respectively after exposure of the worms for 2,7 and 14 days.Both showed close dose-effect relationship.In natural soil,partial relationship was found between Cu content and levels of GSH and MDA in the worms with correlation coefficients being -0.830 (p< 0.001) and -0.599 (p< 0.05),respectively,and between Cd content and levels of GSH and MDA in the worms as well,with correlation coefficients being -0.697 (p< 0.05) and -0.690 (p< 0.05),respectively.Moreover,it was also found that GSH level in the worms was more sensitive than MDA level to soil heavy metals.