Abstract:In order to explore correlations between biosorption and extraction of phenanthrene in soil with different extractants (acetone, dichloromethane, butanol, ethyl acetate and 2-Hydroxypropyl-beta cyclodextrin, HPCD), phenanthrene in soils different in aging were extracted with mild solvent extraction methods. A significant relationship was observed between the amount of phenanthrene extracted with mild organic extractants (dichloromethane and butanol) and with mild HPCD and phenanthrene accumulation in earthworms (p < 0.05). The mild butanol extractant can be used to better predict bioavailability of phenanthrene in both aged and unaged soils as compared to the other mild extractants in the present study.