A pot experiment with fortified soils was carried out exploring effect of chlorpyrifos on growth of Brassica chinensi, degradation rate of the pesticide in different culture soils, and its uptake and translocation in the plant, so as to provide a theoretical basis for risk assessment of its residue in vegetables and production of safety vegetables. Results show that chlorpyrifos high in concentration (>50.0 mg kg^-1) inhibited significantly growth of the plant, as compared to control. Chlorpyrifos differed in half-life in soil between treatments, ranging from 23.03 to 77.43 d, and its residue in the soils could be absorbed by the roots of Brassica chinensi and translocated to shoots. With the pesticide in the soil rising in concentration, its residue in vegetable root, stem and leaf increased, being the highest in the roots and the least in the leaf. Amount of chlorpyrifos residues in root was the maximum, but in leaf was minimum. The residues of chlorpyrifos in vegetable root, stem and leaf displayed a significant positive linear relationship with the primary residue in the soil, which could be expressed in the following equations: C_root= 0.0251C_soil- 0.2358，C_stem = 0.0123C_soil - 0.0517，Cleaf=0.0007C_soil+ 0.0115. To realize end-to-end control of pesticide residue in vegetables from from field to table, and to ensure production and supply of hazard-free safety vegetables, it is critical to monitor pesticide residues in the soils of the production bases for control at the source.