Quality evaluation of cultivated land is important to guidance of agricultural production. Whether the evaluation method is appropriate or not directly affects reliability of evaluation results. Through practical investigation, collection and analysis of samples, attribute data of 249 surface soil samples and rice yield data of 95 paddy fields were obtained in the study area of Yujiang County, Jiangxi Province. Norm value method and the newly established Bio-Norm method were used separately to determine indicators for evaluation of cultivated land quality and their weights and membership functions as well, which were then used to evaluate cultivated land quality of paddy fields in the study area. Comparison between Bio-Norm method and Norm value method shows that the indicators selected with the Bio-Norm method were more representative than those done with the Norm value method; their weights better reflected importance of the indicators; and the thresholds of their membership functions better reflected the actual requirements of rice growth. Evaluation results show that regression coefficient of determination (R2) between cultivated land quality index(IFI value) obtained with the Bio-Norm method and rice yield was way higher than that with the Norm value method, and the distribution of values obtained with the Bio-Norm method tallied better with the distribution of rice yield grades in the region. Therefore, the Bio-Norm method is more scientific and reasonable than the Norm value method in evaluating cultivated land quality.