Sustainability evaluation of regional cultivated land resources is important to guidance of agricultural production. With the aid of GIS techniques, like overlay analysis and multi-factor comprehensive weighing method, suitability evaluation of the cultivated land resources of the Poyang Lake region was conducted using 11 evaluation indices selected from the aspects of resource, environment, economy and society, based on the applicability assessment, field survey and data statistics. Results show that as a whole, the cultivated land resources of the region is quite high in utilization sustainability level, with 69.05% being high or moderate and only 5.23% being extremely low or un-sustainable, indicating that the region is strong in agricultural productivity, good in agricultural eco-environment and high in agricultural economic level. The findings of the study may be used as scientific basis for exploitation and protection of the cultivated land resources and restructuring of the agriculture of the region.