A greenhouse pot experiment was carried out to study effects of a kind of bioorganic fertilizer (BOF) prepared out of fermentation of organic manure using as a starter strain XZ-173 lipopeptide-producing bacteria that are obviously effective in inhibiting the pathogen causing tomato bacterial wilt (TBW). Results show that the BOF effectively reduced the incidence of TBW with a relative control efficiency being 56.8%. Compared with treatments using chemical fertilizers or organic manure without using XZ-173 as starter, Treatment BOF significantly increased chlorophyll content of tomato leaves, biomasses of roots and shoots, and counts of bacteria and actinomycetes in rhizosphere soil, but reduced population of rhizospheric fungi. These beneficial effects of the BOF give it a broad prospect for application in cropping.