Strains of biuret-degrading bacteria GW-1 were successfully isolated and labeled with green fluorescent protein with the triparental mating method and then the GFP-tagged strains were named GW-1-GFP. The expression of GFP in Strain GW-1-GFP was visualized under the fluorescent microscope and its dynamics was analyzed and stability tested. It was found that heterogenous plasmid did not bring any adverse effect to growth of the host strain GW-1-GFP and the strain was stable. HPLC analysis did not show any significant difference (p<0.05) between the origin stain GW-1 and the tagged strain GW-1-GFP in biuret-degrading ability. The tagged strain GW-1-GFP, proved by the antibiotics resistance screening and fluorescence tracing, could colonize well in the soil and was found to have degraded 50% of the biuret in soil in all the treatments on D25 after the application of Strain GW-1-GFP. It was also found in the experiment that the tagged strain was safe to the environment and vanished from the soil after 45 days. Good colonization of Strain GW-1-GFP in the rhizosphere of wheat seedlings which emerged from wheat seed inoculated with Strain GW-1-GFP was observed and the tagged strain could alleviate the toxic effects of biuret on wheat to a certain extent. The study provides a simple, intuitive method to trace functional microbial agents and corroborate their ecological effectiveness and affinity with crop roots in the soil.