【Objective】Arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) are important members in the soil microbial community, capable of remediating soils polluted with toxic organics, and China has large tracts of soils contaminated with pesticides and some other toxic organic substances, waiting to be remediated. It has been proved that AMF and PGPR can degrade toxic organic matters, however, little has been reported so far about AMF or/and PGPR degrading residues of organophosphorus pesticides in soil. The purpose of the present study was to evaluate efficiency of AMF and PGPR degrading residues of organophosphorus pesticides in soil and to remedy organic pesticide polluted soils with the two groups of soil microbes. 【Method】A pot experiment, designed to have a total of 48 treatments with concentration of methamidophos (0, 50, 100 and 150 µg g^-1) and inoculation pattern of AMF and PGPR (inoculating tomato seeds with AMF Glomus mosseae (Gm), Glomus etunicatum (Ge), and tomato seedlings with PGPR Bacillus subtilis (Bs), Bacillus sp. B697 (Bsp), Pseudomonas fluorescens (Pf), Gm+Bs, Gm+Bsp, Gm+Pf, Ge+Bs, Ge+Bsp, or Ge+Pf), was conducted on tomato (Lycospersicon esculentum, Jinguan in variety) under greenhouse. Colonization of AMF and/or PGPR, and biomass of plant growth (such as plant height, dry weight of shoots per plant, and dry weight of roots per plant) was measured, and methamidophos concentrations in the pots and in the shoots of the plants were determined with gas chromatography. 【Result】Results show that Gm significantly promoted PGPR colonization in the root zone soil and roots, while Pf did significantly AMF colonization, suggesting that Gm and Pf are mutually promoted in colonization. In the pots 100 µg g^-1 in methamidophos concentration, the plants in the pots inoculated with Gm+Pf were much higher than those in the other pots in plant height and in dry weight of shoots as well (except for those inoculated with Ge+Pf), and significantly higher, too, than those in control and those inoculated with PGPR and Ge in dry weight of roots. The plants in the pots inoculated with Gm+Pf were much lower than those in all the other pots in methamidophos concentration in roots and in methamidophos concentration in shoots as well (except those in the pots inoculated with Gm+Bs, Gm+Bsp and Ge+Pf). Inoculation of AMF, PGPR, or AMF+PGPR significantly reduced methamidophos concentration in the tomato plants as compared with those in the control. AMF and/or PGPR could degrade methamidophos residue and the effects of dual inoculations were higher than those of single inoculations. Inoculation with Gm+Pf significantly reduced methamidophos concentration in the root zone soil, through mineralizing the substance by 52%~60.6%, when the concentration of methamidophos in the pots was in the range of 50~100 µg g^-1. Analysis of the dynamics of methylamine dehydrogenase activity in the root zone soil, reveals that inoculation with AMF, PGPR or AMF+PGPR could enhance enzyme activity, and inoculation with Gm+Pf demonstrated the highest enzyme activity. 【Conclusion】It is, therefore, suggested that both AMF and PGPR could promote degradation of methamidophos residue in the soil, and the inoculation of Gm+Pf is the optimal combination under the conditions of this experiment.