Effects of Extraneous Nitric Oxide and Salicylic Acid on Physiological Properties of Wheat Seedlings under Salt Stress
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National Key Research and Development Support Program of China (No. 2017YFD0201705), the National Basic Research Program of China (No. 2015CB150404), and the Indigenous Innovation Project of Shandong Province (No. 2014ZZCX07402)

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    Abstract:

    【Objectives】Wheat is one of the most important crops cultivated in coastal saline soils. The seed germination period and seedling stage are two important growth stages in the life circle of wheat and the plants during the two stages are pretty sensitive to salt stress. This study was designed to explore effects of extraneous nitric oxide (NO) and/or salicylic acid (SA) applied alone or in combination, on growth and physiological properties of wheat under the stress of 120 mmol•L-1 NaCl in an attempt to evaluate the effects of extraneous NO and SA mitigating salt stress on growth of wheat seedlings, and to provide a theoretical basis for elucidating the mechanism of extraneous NO and SA enhancing salt tolerance of wheat. 【Methods】A hydroponic experiment was carried out, cultivating wheat (Triticum aestivum L.) and using extraneous sodium nitroprusside (SNP, a nitric oxide [NO] donor) and SA as regulatory substance to explore effects of extraneous NO and/or SA on growth and physiological properties of wheat under salt stress (120 mmol•L-1 NaCl). This experiment was designed to have six treatments; i.e. CK (Hoagland nutrient solution), NaCl (120 mmol•L-1NaCl), SA (120 mmol•L-1 NaCl+100 μmol•L-1 SA), SNP (120 mmol•L-1 NaCl+100 μmol•L-1 SNP),1/2 (SA+SNP) (120 mmol•L-1 NaCl+50 μmol•L-1 SA+50 μmol•L-1 SNP, and SA+SNP (120 mmol•L-1 NaCl+100 μmol•L-1 SA+100 μmol•L-1 SNP). Growth indices, root activity, photosynthetic pigment content, superoxide anion (O2•-) production rate, H2O2 content, MDA content, electrolyte leakage and antioxidase activities of the wheat seedlings in all the treatments were analyzed for comparison to explore effects of NO and/or SA. 【Results】Results show that Treatment NaCl was significantly higher in O2-1 production rate and H2O2content, but significantly lower in wheat growth and photosynthetic pigment synthesis. Treatment SNP or SA significantly mitigated the damage of wheat caused by salt stress, but the effect of Treatment SA+SNP was more significant. Compared with Treatment NaCl, Treatment 1/2(SA+SNP) was 74.26% higher in root activity and 39.58%, 24.46% and 33.06% higher in content of Chl a, Chl b and Car respectively, but 30.52% and 29.16% lower in O2-1 production rate in leaves and roots and 15.78% and 24.23% lower in content of MDA in leaves and roots, respectively. Besides, Treatment 1/2(SA+SNP) also significantly increased the content of antioxidase as well as the absorption of mineral elements, such as N, P, K and Ca. 【Conclusion】Compared with Treatment SNP or SA, Treatment SA+SNP is more effective in reducing ROS accumulation induced by salt stress, MDA content and electrolyte leakage, improving chlorophyll content, proline content and soluble protein content, antioxidase activity; enhancing root activity in absorbing mineral elements, as well as in inhibiting Na uptake and reducing Na content in the plants, thus relieving salt stress of the wheat seedlings. Among the treatments in the experiment, Treatment 1/2(SA+SNP) is the most effective in mitigating salt stress on wheat.

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ZHANG Qian, HE Mingrong, CHEN Weifeng, DAI Xinglong, WANG Zhenlin, DONG Yuan-Jie, ZHUGE Yuping. Effects of Extraneous Nitric Oxide and Salicylic Acid on Physiological Properties of Wheat Seedlings under Salt Stress[J]. Acta Pedologica Sinica,2018,55(5):1254-1263.

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History
  • Received:January 23,2018
  • Revised:May 02,2018
  • Adopted:June 15,2018
  • Online: June 25,2018
  • Published: