Impacts of Arbuscular Mycorrhizal Fungus Enhancing Abelia grandiflora ‘Francis Mason’ Cd Uptake on Rhizospheric Fungal Communities
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    Abstract:

    [Objective] As a pollutant element generated from industrial and agricultural activities, cadmium (Cd) has become an intensifying environmental problem throughout the world. In order to investigate effects of arbuscular mycorrhizal fungi (AMF) on green seedling Cd uptake, a pot experiment was conducted.[Method] A total of twelve species of green seedlings were planted in the Cd-contaminated pots, separately, with or without AMF inoculation. Particular attention was given to the seedlings of A. grandiflora. Effects of inoculation with AMF on population of the microbes and AMF community structure in the rhizosphere of A. grandiflora were analyzed using the PLFA and Hiseq techniques.[Result] After six months, the twelve species of seedlings ranged from 0.25 to 2.59 mg·kg-1 in leaf Cd concentration, and exhibited an order, excluding Ligustrum japonicum ‘Howardii’ and Eurya emarginata (Thunb.) Makino, of Camellia sasanqua Thunb. < Osmanthus fragrans(Thunb.)Lour < Ligustrum quihoui < Ligustrum sinense ‘Variegatum’ < Elaeagnus pungens var. varlegata Rehd. < Distylium buxifolium < Weigela florida cv. Red Prince < Photinia×fraseri Dress < Abelia grandiflora ‘Francis Mason’ < Weigela florida cv. Variegata. The group inoculated with AMF was obviously higher than their respective ones in the control group without AMF inoculation in leaf Cd concentration. Compared with the control of the same plant, A. grandiflora inoculated with AM2 (Glomus mosseae, BGCAM00164) was 147.85% higher in leaf Cd concentration, so A. grandiflora was designated for investigation in ongoing researches. Twelve months after inoculation with AM2, the plant had Cd concentration in its root, stem and leaf increased up to 164.7, 22.86 and 10.57 mg·kg-1, or 2.64, 2.06 and 1.76 times as high as that in the control, making up a total of 5 078 μg·plant-1, higher than that of the control, which is only 1 745 μg·plant-1. Apparently AM2 reduced the translocation factor and fixed more Cd in the root, thus mitigating Cd damage to the plant. The inoculation of AMF increased PLFA biomass of the AMF, but reduced that of the bacteria and fungi in rhizosphere soil. Glomus, Paraglomus and Archaeospora were the phyla of fungi relatively high in richness in all the samples. Glomus accounted for 55% of the total fungal population, and hence the chief dominant group in the rhizosphere of A.grandiflora. In treatments AM2, Glomus increased significantly from 61.8% to 77.4%, while the AMF community, as a whole, decreased in diversity and richness in the rhizosphere soil, whereas in treatments AM1, Paraglomus increased from 13.1% to 17.8%, but Glomus did not change much. Principal component analysis shows that the AMF community was altered by Cd contamination and AMF inoculation in the rhizosphere of A.grandiflora.[Conclusion] Generally speaking, inoculation with Glomus mosseae may improve the efficiency of A. grandiflora phytoremedying Cd contaminated soils. This type of combined remediation technology can expand the scope of phytoremediation of Cd contaminated soils.

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LIU Chen, GUO Bin, LIN Yichen, FU Qinglin, LI Ningyu. Impacts of Arbuscular Mycorrhizal Fungus Enhancing Abelia grandiflora ‘Francis Mason’ Cd Uptake on Rhizospheric Fungal Communities[J]. Acta Pedologica Sinica,2021,58(2):495-504.

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History
  • Received:November 09,2019
  • Revised:February 08,2020
  • Adopted:
  • Online: February 02,2021
  • Published: March 11,2021