The nutrient concentrations, microorganisms and enzyme activities of soil in pure Eucalypyus urophylla, Acacia mangium, Tectona grandis, Taxodium distichum stands and in mixed stands of Castanopsisfissa and Pinus elliottii, Schima wallichii and Pinus elliottii, Castanopsis fissa and Pinus caribaea were studied. The results show that compared with P. massoniana stand, A. mangium, T. grandis, T. distichum stands and mixed stands with conifer and broadleaf tree could increase organic matter content and improve the nutrient status of soil, whereas the effect of E. urophylla changed with nutrient type. The microorganism amount of P. massoniana stand soil was bigger than those of mixed stand soil of C. fissa and P. elliottii and pure stand soil of E. urophylla, but it was smaller than those of other stand soils. Except for E. urophylla stand soil and A. mangium stand soil, the enzyme activities of other stand soils were bigger than those of P. massoniana stand soil. In a word, E. urophylla was not good at improving soil fertility, whereas other stands could improve soil fertility.